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cdk 161/vol.35 no.2 Mar-Apr 2008
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Cloned embryos generated by
Somatic Cell Nuclear Transfer
(SCNT) technique are good re-
sources for Embryonic Stem Cell
(ESC) with pluripotent characte-
ristics. SCNT becomes a poten-
tial method used in therapeutic
and regenerative medicine with
the aim to produce cells for au-
tologous transplantation. An
advanced method recently deve-
loped to generate inner cell mass
lacking intact trophectoderm
function by targeting Cdx2 gene
by RNAi (RNA interference) is
known as Altered Nuclear Trans-
fer (ANT). Through ANT technique
ethical problems encountered in
SCNT could be resolved for the
reason that the cells created are
not intact embryos. This review
covers briefly the latest methods
used in SCNT including the ANT
technique.
Keywords: ANT, SCNT, Trophec-
toderm, RNAi, Cdx2
Cermin Dunia Kedokt.2008; 35(2): hal 61-3.
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Stem cell has been an interesting
and controversial focus among
researchers and public because
of their potential on cell based
the-rapy. Regarding ethical is-
sues surrounding the embryonic
stem cell source, adult stem cell
becomes a promising alterna-
tive choice. Mesenchymal stem
cell derived from umbilical cord
blood has been a preference for
researchers as recognized for its
high plasticity and low immunoge-
nicity.
With proper treatment, mesen-
chymal stem cell can be grown
into different kind of cells, ready
to be used for cell based therapy.
For that purpose, there has been
many attempts in establishing
culture protocol for mesenchy-
mal stem cell in xeno-free culture
condition to reduce potential
animal pathogen transfer to hu-
man.
Keywords : Stem cell, adult stem
cell, mesenchymal stem cell, cell
based therapy.
Cermin Dunia Kedokt.2008;35(2): hal 64-7
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The discovery of endothelial pro-
genitor cells (EPC) has major im-
plications in angiogenic therapy.
The limited numbers of EPC found
in adult blood circulation has been
the main obstacle for the usage
of EPC. Various attempts have
been made to expand EPC in vi-
tro in order to get sufficient num-
bers of EPC required for human
therapy. This review summarizes
the characteristics and different
methods used in the expansion
of EPC. Several methods for EPC
quantification and surface mark-
ers used for EPC identification
will also briefly covered.
Key words : EPC, stem cell, expan-
sion, Endothelial Progenitor Cell.
Cermin Dunia Kedokt.2008;35(2): hal 68-71
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