English Summary
THE INVESTIGATION ON ANTI-
MUTAGENIC AND ANTIOXIDANT
ACTIVITY OF SCHIMA WALLICHII
KORT. LEAVES
Didi Jauhari Purwadiwarsa, Anas
Subarnas, Cucu Hadiansyah,
Supriyatna
Department of Pharmacy and Biology,
Faculty of Mathematics and Physics,
Padjadjoran University, Bandung,
Indonesia
An investigation on antimuta-
genic and antioxidant activity of
the butanol fraction of Schima
wallichii Korth leaves has been
carried out. The experiment of an
in vivo antimutagenic activity using
a micronucleus test showed that
butanol fraction used orally de-
creased the frequency of micro-
nucleated polychromatic cell
erythrocytes (MNPCE) from the
bonemarrow smears of Swiss-
Webster male mice elevated by
cyclophosphamide at a dose of
50 mg/kg intraperitoneally. The
butanol fraction at a dose of
300 mg/kg decreased the
frequency of MNPCE by 10,5%
while at a dose of 600 mg/kg
decreased by 38,27%.
An in vitro antioxidant activity
using nitroblue tetrazolium (NBT)
method showed that butanol frac-
tion inhibited the reduction of NBT
by superoxide generated by the
xanthine oxidase system. The in-
hibition by butanol fraction at a
concentration of 200 µg/ml was
68,66% while at a concentration
of 400 µg/ml was 94,37%.
The result indicated that the
bu-tanol fraction had antimuta-
genic and antioxidant activities.
Cermin Dunia Kedokt. 2000; 127: 18-21
djp, as, ch, s
EFFECTS OF EXPIRED AMPICILLIN
PRODUCT ON THE GROWTH OF E.
COLI IN VITRO
Raharni*, Sugeng Riyanto**,
Koesniyo***
* Pharmacy Research and
Development Centre Health Re-
search and Development Cen-
tre, Department of Health,
Jakarta, Indonesia
** Faculty of Pharmacy, Gajah
Mada University, Yogyakarta
Indonesia
*** Faculty of Medicine. Gajah
Mada University, Yogyakarta,
Indonesia
The purpose of the study is to
determine the correlation between
the length of expiration date of
ampicillin products and the poten-
cy to inhibit E. coli growth, com-
pared to the standard ampicillin.
Using dilution method, Minimal
Inhibition Concentration (MIC) and
Minimal Bactericidal Concentra-
tion (MBC) of several different
expired ampicillin products
against E. coli are determined.
The results indicate that MIC
and MBC of the expired ampicillins
are lower than the standard ampi-
cillin.The longer the expiration date
of ampicillin have been passed
the smaller the potential against
the growth of E. coli.
Cermin Dunla Kedokt. 2000; 127: 41-4
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RESISTANCE OF M. TUBERCULOSIS
TO THE PURE AND THE GENERIC
ANTITUBERCULOSIS DRUGS IN THE
DEPARTMENT OF CLINICAL PA-
THOLOGY, FACULTY OF MEDICINE
PADJADJARAN UNIVERSITY/ DR.
HASAN SADIKIN GENERAL HOS-
PITAL, BANDUNG
Hotman Sinaga, Idaningroem
Sjahid, Monang Siahaan, Ida
Parwati Santoso
Department of Clinical Pathology,
Faculty of Medicine, Padjadjaran
University, Dr. Hasan Sadikin General
Hospital, Bandung, Indonesia
The inappropriate treatment of
tuberculosis may result in drug re-
sistance that is more difficult to
treat. Proper treatment should be
based on susceptibility test; but this
test is not easily performed and
also expensive. So it is necessary
to find cheaper and easier ob-
tainable reagent and method.
A comparative study on the sus-
ceptibility test on 50 isolated of M.
tuberculosis using pure and gene-
ric antituberculosis drugs as media
was carried out in the Department
of Clinical Pathology, Faculty of
Medicine, Padjadjaran University/
Hasan Sadikin General Hospital.
This study revealed that the
result was not significantly different
(p > 0,05) and both methods
have a 100% accuracy.
Generic antituberculosis drugs
can be used for the susceptibility
test of M. tuberculosis.
Cermln Dunla Kedokt. 2000; 127: 49-53
hs, is, ms, ips
Cermin Dunia Kedokteran No. 127, 2000
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