English Summary
EVALUATION OF LIVER FUNCTION
AMO PATIENTS TREATED WITH
OAT
Zulkarnain Arsyad
Dept. of Internal Medicine, Faculty of
Medicine, Padjadjaran University, Ban-
dung, Indonesia.
The administration of anti
tuberculosis drugs may cause
various side effects including
liver function disorders.
The study on the tuberculosis
out patients had been carried
out to evaluate liver function
disorders caused by anti tuber-
culosis drugs from September
1993 to December 1993. Thisstudy
found 28% elevation of SGOT,
SGPT and Alkali Phosphatase
value among 1 & 2 months-
treated patients, 27% among 3 &
4 months-treated patients, and
57% among 5 & 6 months-treated
patients. The elevation of SGOT,
SGPT and Alkali Phosphatase
were not more than twice of
normal value. The liver function
disorders was also influenced by
anemia and old age. Regular liver
functions monitoring was re-
commended for tuberculosis pa-
tients which were treated by anti
tuberculosis drugs, especially
when accompanied by anemia
and old age.
Za
Cermin Dunia Kedokt. 1996; 170: 15-8
LIVER DISEASES IN PREGNANCY
AB Wardoyo
Internist, Semarang, Indonesia
Various liver diseases during
pregnancy have been discussed.
The jaundice in pregnant
woman may be peculiar to preg-
nancy, such as acute fatty liver,
toxaemias, intrahepatic chole-
stasis and hyperemesis gravi-
darum; or as intercurrent jaun-
dice during pregnancy, such as
viral hepatitis, gallstones, hepa-
totoxic drugs and underlying
cirrhosis.
The most common cause of
jaundice in pregnancy is viral
hepatilis, then followed conse-
cutively by intrahepatic chole-
stas and gallstones.
Several changes in liver func-
tion during pregnancy have to
be detected, especially in third
trimester, such as increased alka-
line phosphatase, globulin and
cholesterol; and decreased al-
bumin level.
Abw
Cermin Dunia Kedokt. 1996; 110:21-5
THALASSEMIA DIAGNOSIS WITH
POLYMERASE CHAIN REACTION
Sunarto
Dept of Child Health. Dr. Sardjito General
Hospital/Gajah Mada University, Yogya-
karta. Indonesia.
The clinical diagnosis of tha-
lassemia syndrome is not difficult
to establish with relatively simple
investigations, but molecular
study is needed to determine the
genotype and for the prenatal
diagnosis. In the molecular study,
the DNA should be first amplified
and among the amplification
techniques polymerase chain
reaction is the simplest and the
most rapid one. The procedure
can in vitro amplify a DNA seg-
ment million times. It has been
used extensively in the molecular
studies.
While amplifying a DNA seg-
ment, polymerase chain reaction
can detect gene deletion di-
rectly. A procedure based on
polymerase chain reaction prin-
ciples using allele specific oligo-
nucleotide primer(s), called
amplification refractory mu-
tation system (ARMS), can de-
tect mutant(s) directly. Reverse
dot blot hybridization on poly-
merase chain reaction product
using allele specific oligonucleo-
tide probe(s) has many advan-
tages. Either ARMS or RDB proce-
dure, both have very high sensi-
tivity and specificity, need only a
short time, and are relatively
inexpensive. Using appropriate
allele specific oligonucleotide(s),
either as probe(s) or primer(s),
commonly occured mutants in a
certain population can be de-
tected easily.
S
Cermin Dunia Kedokt. 1996;110: 26-31
Cermin Dunia Kedokteran No. 110, 1996
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